Automation of a Hemagglutination-Inhibition Test for Parainfluenza 3 Antibodies in Bovine Sera

Automation of a hemagglutination-inhibition test for parainfluenza 3 antibodies in bovine sera.

An automated hemagglutination-inhibition (HI) test for the "shipping fever" strain (SF-4) of parainfluenza 3 antibody in bovine sera was developed and compared to manual tube and microtiter test procedures. The automated system operating at 60 samples per hr provided the most test results per specified time period, and the manual tube test provided the least. The manual microtiter test and the ...

Serological survey for antibodies against infectious bovine rhinotracheitis and parainfluenza 3 viruses among cattle in Iran

Hepatitis A virus hemagglutination and a test for hemagglutination inhibition antibodies.

Like enteroviruses, hepatitis A virus (HAV) hemagglutinated various species of erythrocytes under similar conditions. HAV-specific antibodies in both acute- and convalescent-phase sera were found to inhibit hemagglutination. The HAV hemagglutination inhibition test can be used for diagnosis, epidemiological surveillance, and vaccine assessment.

Preparation of an Inactivated Egg Drop Syndrome Antigen for Using in Hemagglutination Inhibition Test.

To prepare hemagglutination inhibition (HI) antigen the egg drop syndrome virus (EDSV) was propagated in 10-day-old embryonated duck eggs. The virus antigen was inactivated with two methods including heating (65°C) and adding 0.5% formaldehyde by considering low destroying effect on hemagglutination (HA) and HI titers. The EDSV HI titers of 310 sera and 100 yolks obtained from 23 chicken farms ...

antibodies to myxovirus parainfluenza-3 virus in human sera in iran

199 human serum samples were examined for the presence of myxovirus parainfluenza 3 (mpi 3)haemagglutination inhibition (hi) antibodies. or tbe total male sera, 75% and female 70.52% had hi antibody titers of t:32 or greater. the percentage of positive samples increased according to the age grouping of the human beings. these results indicate that mpi 3 virus infection is widespread among the h...